ABSTRACT
AIM:To study the chemical constituents of Erigeron Injection by HPLC. METHODS: Under the guidance of HPLC analysis,two constituents were separated and purified by the aid of column chromatography with silica gel,and identified by UV、 IR、 MS、 NMR respectively and documentary data. RESULTS: Two compounds from semi-finished products of Erigeron Injection were identified as caffeic acid(Ⅰ) and chlorogenic acid(Ⅱ). CONCLUSION: Two main peaks in HPLC chromatogram of Erigeron Injection are Ⅰ and Ⅱ.
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AIM: To compare the chemical constituents of Injectio erigerontis and breviscapine. METHODS: High performance liquid chromatography and diotron array detection (HPLC-DAD) was adopled with Hypersil ODS2 Column (250 mm?4.6 mm,5 ?m), and mobile phase was acetonitrile-H 2O-H 3PO 4(18∶82∶0.2),detection wavelength was at 286 nm, temperature was at 40 ?C. RESULTS: Weak peaks of Seutellarin and caffeic acid were resembling between injetio erigerontis and breviscapine in UV spectra. but Injectio eriperontis possibly contained 10 catteic acid derivatives. CONCLUSION: Injectio erigerontis and breviscapine are completely different in chemical constituents,though their clinic application and curative effect are equal.
ABSTRACT
Aim To develop a simple and rapid method to monitor insulin receptor kinase activity and provide a novel cell-based model for screening anti-diabetes drugs.Methods CHO cells were co-transfected by plasmids which respectively contained insulin receptor gene,STAT5b gene and luciferase gene driven by STAT5 response elements.The expression of exogenous gene in transfected cells was examined by RT-PCR.The transfected cells were treated by insulin,and then the concentration and time-dependent response of luciferase expression to insulin induction was examined.Moreover,the specificity was identified by AG1024 treatment and PTP1B gene transfection.Results Expressions of insulin receptor and STAT5b were detected in the transfected CHO cells.The expression of luciferase in transfected cells was induced by insulin in concentration and time-dependent way.The maximal induction fold was 6.25.Moreover,the inducible expression of luciferase by insulin could be specifically blocked by tyrphostin AG1024,an inhibitor of insulin receptor kinase,or co-transfected PTP1B gene.Conclusions The insulin receptor kinase activity can be detected by expression of reporter gene with high sensitivity and specificity in this cell model,and with potential value in high throughput screening for insulin receptor activators and sensitizers.